Insertion junction: LMJ.RY0402.210386_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.210386
Chromosome:chromosome 3
Location:2869257
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g162701 SRH5 SNF2-related DNA/RNA helicase; (1 of 1) 3.6.4.11 - Nucleoplasmin ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTCAATGACGACGGGCGCTGGCGGGAG
Internal bar code:TCACGGCTGGGGTATGGCGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTCTTCCTCCTCCTCCT
Suggested primer 2:AAATCCTCTCGCCCCTTTTA