| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210390 |
| Chromosome: | chromosome_12 |
| Location: | 6516516 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g538750 | LSM1 | U6 snRNA-associated Sm-like protein LSm1 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTCCTTTCGCCCCGTTTTCCAGCAGTCAG |
| Internal bar code: | GAGGCACGCGGTGGCATCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 99.7601 |
| LEAP-Seq n confirming: | 7900 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCATTGAAGGAGCTGTG |
| Suggested primer 2: | AGGGTGCGTGCTACTGACTT |