Insertion junction: LMJ.RY0402.210406_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.210406
Chromosome:chromosome 2
Location:2719657
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCGTCCCTGTTGACAGCTGCCCTCGAG
Internal bar code:CTGTGCCGGTCGGCCACCGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:98.8881
LEAP-Seq n confirming:1334
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCATTCTTGCGCTCTGAT
Suggested primer 2:CAGTGAGTCCTAGCCAAGCC