Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.210406 |
Chromosome: | chromosome_16 |
Location: | 6911465 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g676645 | FAP200 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTATGATGCAGGCGCGGCGGGGCTCTGCA |
Internal bar code: | GGCTTGGCACGGCCGGCAGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 327 |
LEAP-Seq percent confirming: | 99.5604 |
LEAP-Seq n confirming: | 453 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATCGCAGTCGTCATCAAGC |
Suggested primer 2: | CCTCTCAGACCAGCTCCATC |