| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210412 |
| Chromosome: | chromosome 5 |
| Location: | 1766068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242400 | PGR5 | Proton-gradient related; (1 of 1) PTHR35709:SF1 - PROTEIN PROTON GRADIENT REGULATION 5, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGATTGTTACATCATGCACCTTCTTGC |
| Internal bar code: | TTAAACAAGATCCGAGCCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 99.4042 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGGACGGTGTCTTTTGA |
| Suggested primer 2: | GCTATGGCTTGGTGGTGTTT |