Insertion junction: LMJ.RY0402.210419_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210419
Chromosome:chromosome 3
Location:7351282
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACGCACCTCGAGTGTGCGCGCCAGTAGC
Internal bar code:TTTCGCCGGCAGCCACCCTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:99.5763
LEAP-Seq n confirming:235
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCCCTCCACCTCTGTCTT
Suggested primer 2:AAAGATTGACACGGACAGGC