| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210425 |
| Chromosome: | chromosome 12 |
| Location: | 1273674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489250 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGCTGGGCATGGAGGTAGGCCACAGTC |
| Internal bar code: | TCGTCGGGCGAATGGGCCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.8983 |
| LEAP-Seq n confirming: | 2948 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATTTGCCATCTTGCTCA |
| Suggested primer 2: | AGCACGAGTCATTCGGACTT |