| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210431 |
| Chromosome: | chromosome_7 |
| Location: | 1953635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g325751 | FAP258 | Similar to Flagellar Associated Protein FAP258 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAACTCCTTCTGCTCGCTTTCCTCCCATCC |
| Internal bar code: | ACCCTGGTGAGGTTATTGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 97.9757 |
| LEAP-Seq n confirming: | 242 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATTGGTCTTTTGCAAGG |
| Suggested primer 2: | GGAAGAGACGCCATGAATGT |