| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210433 |
| Chromosome: | chromosome 6 |
| Location: | 579924 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253200 | PKY1 | Protein tyrosine kinase; (1 of 1) PTHR23257:SF534 - SERINE/THREONINE-PROTEIN KINASE ROCO7-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGCAACACTTCTGAAAGCAAGCCTGAT |
| Internal bar code: | ATGCCCGACGGCTCATAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 96.8547 |
| LEAP-Seq n confirming: | 4619 |
| LEAP-Seq n nonconfirming: | 150 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCCCAAACTAACAAGGA |
| Suggested primer 2: | GGGTTTCCCTCCAGAGTTTC |