| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210437 |
| Chromosome: | chromosome_1 |
| Location: | 5688377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACTAAGCCTTGCTTGCTCTTTGCGTCCCA |
| Internal bar code: | GAGCCGACCTGCGCGATGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 89.8809 |
| LEAP-Seq n confirming: | 6262 |
| LEAP-Seq n nonconfirming: | 705 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCAAACACACTGCTGAA |
| Suggested primer 2: | GGTTCTGGGTTGCATCACTT |