| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210439 |
| Chromosome: | chromosome 3 |
| Location: | 1811120 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154350 | COX2A | Cytochrome c oxidase subunit II, protein IIa of split subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACCATCTAGCCAGCGAGAGCACCGGC |
| Internal bar code: | CCGCTGGGCTTCACCGTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 98.9749 |
| LEAP-Seq n confirming: | 1738 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACCCTCATCTACTCGC |
| Suggested primer 2: | TCGCTCAAAGTCGGAAAGTT |