| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210455 |
| Chromosome: | chromosome 9 |
| Location: | 1755217 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396500 | HAD3 | Halo-acid dehalogenase-like hydrolase; (1 of 98) IPR023214 - HAD-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCAAGCGGTCCGTTGAGTGCCGGGCCA |
| Internal bar code: | ACTCGTGGACACACCCACTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 99.7461 |
| LEAP-Seq n confirming: | 13750 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGAAGGAACACAAACCT |
| Suggested primer 2: | CAAGCTTAAGCCCGTCAATC |