| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210456 |
| Chromosome: | chromosome 1 |
| Location: | 2065364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011100 | CAS1 | (1 of 1) 5.4.99.8 - Cycloartenol synthase / 2,3-epoxysqualene--cycloartenol cyclase; Terpenoid cylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCTCCTCGCCCAGCAGCACCAGGCGC |
| Internal bar code: | AGCCCAGATAGGTTAGCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 93.6718 |
| LEAP-Seq n confirming: | 4041 |
| LEAP-Seq n nonconfirming: | 273 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTTATACCCTGTCCCA |
| Suggested primer 2: | ATCACCTACGCCAACTACCG |