| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210457 |
| Chromosome: | chromosome 10 |
| Location: | 2939545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440350 | (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCATCATCGGGCTGCAGCTTTTGCCAGG |
| Internal bar code: | CTCCTCGGGATGTGTCACTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.186 |
| LEAP-Seq n confirming: | 9870 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTGTAGCTTCATCAAAA |
| Suggested primer 2: | TTTCCGAGTGACCCGTTTAG |