| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210458 |
| Chromosome: | chromosome 17 |
| Location: | 6807233 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744997 | PNO4 | FAD dependent oxidoreductase; (1 of 1) PTHR10617//PTHR10617:SF52 - ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE // OXIDOREDUCTASE/ELECTRON CARRIER-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACTGGCGCGCCGGCGCCTGTGCCTCCC |
| Internal bar code: | TAGGTTAATCAAGGGGTGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 99.5025 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGCAAACTTCCAGTCA |
| Suggested primer 2: | CTGAAACGTCCACCCCTTTA |