| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210464 |
| Chromosome: | chromosome 2 |
| Location: | 634529 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077951 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase; putative ABC family transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGAAGCAGCAGCGCCGGCGCCCCGCC |
| Internal bar code: | CTAGACCTATGAGGTCGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 70.1863 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGCAAACTCACGTTCC |
| Suggested primer 2: | TGCTGCTAGTGTGGGACAAG |