Insertion junction: LMJ.RY0402.210471_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210471
Chromosome:chromosome 3
Location:8708868
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g204465 GT90F36,GT90-36 GT90 family protein 36; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATGACACTCACGTCCGCGACGTCGCTG
Internal bar code:CTATATCAAGGTGTGAATTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:99.7419
LEAP-Seq n confirming:4251
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACTTCAAACCCCCTACGCT
Suggested primer 2:TCTAGGAAACGGCCAAAATG