| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210484 |
| Chromosome: | chromosome 1 |
| Location: | 2960178 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018075 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGACGTCAGCTTAGACACGGGCTGCTG |
| Internal bar code: | GGCTAGTTCATCAGGTACTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.0373 |
| LEAP-Seq n confirming: | 11522 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCTGGCGATAGAGTACA |
| Suggested primer 2: | TTGCGCGCTTATCTATTGTG |