| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210489 |
| Chromosome: | chromosome 7 |
| Location: | 161257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313250 | (1 of 1) 3.6.3.17//3.6.3.25 - Monosaccharide-transporting ATPase // Sulfate-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGATACATCTTCAGGCAAGCCCCTTCCC |
| Internal bar code: | ACACATTGGGACTGCTACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.6768 |
| LEAP-Seq n confirming: | 3393 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCATGTGACCAGTTGC |
| Suggested primer 2: | ACTGCGTTCACCTTTATGGG |