| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210521 |
| Chromosome: | chromosome 11 |
| Location: | 536614 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467603 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCCTACCTTGGGGCTCACGCCGCGTAG |
| Internal bar code: | CACGGAGAATACGGTTGACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 95.9647 |
| LEAP-Seq n confirming: | 3044 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCACCTCTTCGGGAAAAT |
| Suggested primer 2: | ATACACGCACCCACAGCATA |