| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210525 |
| Chromosome: | chromosome 16 |
| Location: | 1125949 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649900 | HTR40,HTR24 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGAATCTCGCGTAGCGCCACAGTTCCG |
| Internal bar code: | GGGCGGGAAAGTGTGAGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.7799 |
| LEAP-Seq n confirming: | 28104 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCAACCATGTGACTACC |
| Suggested primer 2: | CGTCTCCTCGTAGATCAGGC |