| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210540 |
| Chromosome: | chromosome 3 |
| Location: | 3965387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171700 | (1 of 1) 1.2.1.50 - Long-chain-fatty-acyl-CoA reductase / Acyl-CoA reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAACTGGCTCGTCCATGGCAAGGCAGG |
| Internal bar code: | GACATGTGCGAGTTTTTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 99.5517 |
| LEAP-Seq n confirming: | 6440 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGCTACTGCGTGTGTAAG |
| Suggested primer 2: | TTTAGCGCGGGCTTATCTTA |