| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210554 |
| Chromosome: | chromosome 17 |
| Location: | 5678964 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738600 | RLS5 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATACCCCTTCAACCCCCCAATCACAA |
| Internal bar code: | ACCGTGGGCCCTTTCCGAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1330 |
| LEAP-Seq percent confirming: | 99.0137 |
| LEAP-Seq n confirming: | 3915 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACGCTAGGATGCTGGA |
| Suggested primer 2: | GCATAACCTCTCAAGCCAGC |