| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210554 |
| Chromosome: | chromosome 17 |
| Location: | 5678967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738600 | RLS5 | RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTGAGCGAAGCGCTGACTCGTCGTGT |
| Internal bar code: | GCCTCACCTCGCCGTCATGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1136 |
| LEAP-Seq percent confirming: | 99.461 |
| LEAP-Seq n confirming: | 8858 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACGCTAGGATGCTGGA |
| Suggested primer 2: | GCATAACCTCTCAAGCCAGC |