| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210576 |
| Chromosome: | chromosome 11 |
| Location: | 1556775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467756 | MMP5 | Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTTCATGTCGCCGGTGCCCGGTGCCC |
| Internal bar code: | GTGTGATAGCGCCTTTCCTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 99.2159 |
| LEAP-Seq n confirming: | 13540 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAAATGTCCCAGTGTGC |
| Suggested primer 2: | GATGAGGCAGCAGAGCCTAC |