| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210593 |
| Chromosome: | chromosome 13 |
| Location: | 4373393 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602950 | (1 of 1) IPR027050 - Nuclease SbcCD subunit C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACGGCAAAGCCTCTCGCAGTCATCTGC |
| Internal bar code: | TGGGCCCGTGGCTCCCCCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1055 |
| LEAP-Seq percent confirming: | 99.7285 |
| LEAP-Seq n confirming: | 6612 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCTTGGAGCTAAACAC |
| Suggested primer 2: | TAAGTTGCCAAAATCCCTGC |