| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210597 |
| Chromosome: | chromosome 9 |
| Location: | 2588127 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCACGCGGGCAGAAAACAAAAAAACAG |
| Internal bar code: | GTGACCGTGTGTCCAGATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 92.9965 |
| LEAP-Seq n confirming: | 74851 |
| LEAP-Seq n nonconfirming: | 5637 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGGTGTCAGTCTCCCC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |