| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210601 |
| Chromosome: | chromosome 7 |
| Location: | 453732 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315450 | CSG2 | Predicted protein of CSG family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGCCGCGCGGTCGCTGCTGCTATTCG |
| Internal bar code: | CAGTGAAAGCTTTAGGGGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 71.1425 |
| LEAP-Seq n confirming: | 4477 |
| LEAP-Seq n nonconfirming: | 1816 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAAACTCTCGGCTTCGG |
| Suggested primer 2: | GGAGGTAAAACGGTGGGAGT |