| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210606 |
| Chromosome: | chromosome 2 |
| Location: | 715877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGTTCCTCAAGGCGCAGGTGGGAGCGT |
| Internal bar code: | ATAAATTCCGGAAATTGGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.5523 |
| LEAP-Seq n confirming: | 3558 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGTTATGTGGGATGGTC |
| Suggested primer 2: | CAGACATGTGAGGAGAGGCA |