| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210606 |
| Chromosome: | chromosome 9 |
| Location: | 6997325 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410900 | NAR2,NIT8 | nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTAATAATACATGGCGTTCCACTTCCCC |
| Internal bar code: | GGTCACGCGGGATGCACATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 99.715 |
| LEAP-Seq n confirming: | 5949 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGACAAGGACATCGG |
| Suggested primer 2: | GACGTGTATGAACCAGCCCT |