| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210614 |
| Chromosome: | chromosome 1 |
| Location: | 6101546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043550 | (1 of 3) IPR000104//IPR004333 - Antifreeze protein, type I // Transcription factor, SBP-box | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCATCGAAGTGCCGCCCGACTGCTTGT |
| Internal bar code: | AGGAAGGCGGGAGCATTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.6212 |
| LEAP-Seq n confirming: | 13150 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAACTTCATACGCAGCAG |
| Suggested primer 2: | AAGAGAAACCGCCTCACTCA |