| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210626 |
| Chromosome: | chromosome 6 |
| Location: | 8048218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304350 | COX12 | Mitochondrial cytochrome c oxidase subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGTGCAAGACCGTCAAAGCTGAAAGG |
| Internal bar code: | GGAGCCAGTCCCGGCTCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 99.8993 |
| LEAP-Seq n confirming: | 5954 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTGGGGAGTGGATATG |
| Suggested primer 2: | ACAACAATCAAAGGCAAGGG |