Insertion junction: LMJ.RY0402.210627_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210627
Chromosome:chromosome 13
Location:3741839
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCACTGCCTGCCCTCCACACACAGGT
Internal bar code:TTTAGTTATTAGACTCGTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:68.1265
LEAP-Seq n confirming:280
LEAP-Seq n nonconfirming:131
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:ACAAACGAAATTGTGAGCCC