| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210627 |
| Chromosome: | chromosome_16 |
| Location: | 1420710 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g652350 | FAP183 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCTGACAAGCCCGGCTTTGCCGCATGCGA |
| Internal bar code: | GACTCGCGCCAGGTGGTACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.1716 |
| LEAP-Seq n confirming: | 20113 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCATGAGGCAGCTGAAC |
| Suggested primer 2: | CGATTGACGTGATTGGAATG |