| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210629 |
| Chromosome: | chromosome 3 |
| Location: | 6124717 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191800 | (1 of 33) PF13855 - Leucine rich repeat (LRR_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGACCGAATCCACTGCTGCTGAGCGGT |
| Internal bar code: | TCGCGTGGGCCTTCTGCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 929 |
| LEAP-Seq percent confirming: | 99.5817 |
| LEAP-Seq n confirming: | 10476 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCACGCTTGTGTCAAAC |
| Suggested primer 2: | CGTTCCTCGTTATTGTCCGT |