| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210657 |
| Chromosome: | chromosome 10 |
| Location: | 2194121 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACTGGCTGCCGCGCTTGGGCACGGCCA |
| Internal bar code: | GCCCCTCAGGGGTGGGAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 87.0787 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTCCAGCGTCATCAACA |
| Suggested primer 2: | ACCTGGTCCACGATAAGCAC |