| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210663 |
| Chromosome: | chromosome 11 |
| Location: | 1069200 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467674 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACAACCCAATGCAAGCCCACTTCCCGAA |
| Internal bar code: | TTTTTACTGCTCGTCAGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 98.7376 |
| LEAP-Seq n confirming: | 1095 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCGTCGTAAATTGAGAAA |
| Suggested primer 2: | GCTTTGACTTCACCAGCTCC |