| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210676 |
| Chromosome: | chromosome 3 |
| Location: | 868772 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146587 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACACGACGCAATGCATGGAAACCGGG |
| Internal bar code: | ACGGGATTGTATCCTCGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1070 |
| LEAP-Seq percent confirming: | 98.0611 |
| LEAP-Seq n confirming: | 33583 |
| LEAP-Seq n nonconfirming: | 664 |
| LEAP-Seq n unique pos: | 188 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCTACCATATGTGCC |
| Suggested primer 2: | CCTTGCAAGAAGAATCCAGC |