| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210685 |
| Chromosome: | chromosome 1 |
| Location: | 6664709 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047850 | (1 of 1) 4.2.1.145 - Capreomycidine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACCAGCGGGCGGGAATCCGGGAATTAC |
| Internal bar code: | GAGTTTTTTGCAGTGGGCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 58.8786 |
| LEAP-Seq n confirming: | 13903 |
| LEAP-Seq n nonconfirming: | 9710 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTTCAGCTCAGCCACTC |
| Suggested primer 2: | CTTAAGATGTAGCTCGCCCG |