| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210733 |
| Chromosome: | chromosome 3 |
| Location: | 1979803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155800 | (1 of 1) K15025 - probable RNA-binding protein EIF1AD (EIF1AD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGCCCTGCCTGCGCGCCCCTGGCCAA |
| Internal bar code: | ACCGACGCATAGCGGCACGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 99.5627 |
| LEAP-Seq n confirming: | 17305 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGCTATCGTATCGTTGA |
| Suggested primer 2: | TCAGATGGTGCAAGTGCTTC |