| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210739 |
| Chromosome: | chromosome 5 |
| Location: | 933904 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247100 | COPT1,COTH1 | spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACACAATCAAAAGGCCGCACCTTGGG |
| Internal bar code: | CAGTGACCGGGGACTGCTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 49.2117 |
| LEAP-Seq n confirming: | 1592 |
| LEAP-Seq n nonconfirming: | 1643 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTGTGGAGTGGAGTGGC |
| Suggested primer 2: | ACACACACACACACACACGC |