| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210744 |
| Chromosome: | chromosome 10 |
| Location: | 5983055 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462600 | PUS15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGACCGATATCTCAACAACCACCACCAC |
| Internal bar code: | TTGGAATCGTGGGCGCAGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.594 |
| LEAP-Seq n confirming: | 9077 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGTGCTCGTGTAGAAC |
| Suggested primer 2: | GTGTACATTTCCGCCCTCAC |