| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210748 |
| Chromosome: | chromosome 2 |
| Location: | 6944079 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120200 | (1 of 4) 4.1.1.39 - Ribulose-bisphosphate carboxylase / RuBP carboxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAAGCCGCGACCGTCGGCACAACCCGG |
| Internal bar code: | TGGGTCACTGCATAGGATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 72.133 |
| LEAP-Seq n confirming: | 6466 |
| LEAP-Seq n nonconfirming: | 2498 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGTCAAATCTGCAAGC |
| Suggested primer 2: | ACGCAGAGACATTGTTGCAC |