| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210754 |
| Chromosome: | chromosome 10 |
| Location: | 2070580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433100 | OBGE1,OBGE | Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACCAGCTCGATGACGCCCTGCCCGGTG |
| Internal bar code: | CTACGATTCTCGCGTGGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 97.4603 |
| LEAP-Seq n confirming: | 307 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTACCCCTTCACCACGC |
| Suggested primer 2: | GGATGGGTTGCTGTACGAGT |