| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210758 |
| Chromosome: | chromosome_11 |
| Location: | 3377124 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g480451 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAGGTAGACTAGCTAGTGTCGAATCTGTA |
| Internal bar code: | CCAATAAATTCCCCTCGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 4.23977 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 1965 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGGAAACGGGACTTCT |
| Suggested primer 2: | TCATGGCACCCTTTTGTGTA |