| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210768 |
| Chromosome: | chromosome 3 |
| Location: | 4378929 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175351 | LMR2 | (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATATTTACAGGTGGCGTGGGTGGCGGAG |
| Internal bar code: | CGCGGTCGGGATGGTCCTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.3491 |
| LEAP-Seq n confirming: | 1221 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCAAAGTCAAGGATGT |
| Suggested primer 2: | CCAGACTAAGGCAGGTCTCG |