| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210770 |
| Chromosome: | chromosome 16 |
| Location: | 5552695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680200 | (1 of 1) PTHR31419//PTHR31419:SF1 - FAMILY NOT NAMED // AUXIN EFFLUX CARRIER FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCTTCACAGTGAACAGGAACAGCGTC |
| Internal bar code: | GGCTTCAGGCATAGACTTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.6262 |
| LEAP-Seq n confirming: | 4531 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCACCAGATCCCATCTA |
| Suggested primer 2: | CATCCTCTGTCTTGTGCGAA |