| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210788 |
| Chromosome: | chromosome 3 |
| Location: | 3449714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167250 | (1 of 1) PF05673 - Protein of unknown function (DUF815) (DUF815) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGGCTACCCGCGCACGCCGCACGTAGT |
| Internal bar code: | AGCCAACACGCTGCCGGTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 99.0866 |
| LEAP-Seq n confirming: | 6617 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCACAGAATACAAGACGC |
| Suggested primer 2: | GCGCTATCAGGGTTATGGAA |