| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210798 |
| Chromosome: | chromosome_13 |
| Location: | 3734461 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589350 | FBB6 | Flagellar/basal body protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGTTTCCCGTTGCCGCCGTGTGCAGCCGT |
| Internal bar code: | GCCTCCCACTTCTTGTTGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 97.3337 |
| LEAP-Seq n confirming: | 10039 |
| LEAP-Seq n nonconfirming: | 275 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATGGCTCGAGTGTTGGG |
| Suggested primer 2: | GAGGTAGCTGAACTCCACCG |