| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210799 |
| Chromosome: | chromosome 7 |
| Location: | 460499 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315500 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCTCCCTGACCCTCCCTCCCTCCCCTT |
| Internal bar code: | GAGCTACGGCACGGGCATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 43.5691 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 351 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGGTTGTCGTCGTTATT |
| Suggested primer 2: | AGTATAGCAGGCGCGTTGTT |